Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School
Our long-term goal is to understand transport mechanisms in the cell. To do this we use a combination of structural, biophysical and biochemical techniques with an emphasis on high-resolution electron microscopy.
We are interested in understanding transport mechanisms in the cell. In particular, we are interested in how molecules are transported into, out of, and within cilia. Cilia (also known as flagella) are finger-like organelles that project from the surface of almost all eukaryotic cells. Motile cilia produce a driving force for locomotion or fluid flow whereas immotile cilia are involved in developmental signaling and sensory perception. Genetic disorders, collectively known as ciliopathies, can affect both types of cilia and lead to infertility, ectopic pregnancy, respiratory disease, blindness and polycystic kidney disease, among others. As treatment for ciliopathies is predominantly palliative, understanding how cilia are made and what goes wrong in ciliopathies is of vital importance.
To help understand cilia and their dedicated transport mechanisms, we use a combination of structural, biophysical and biochemical techniques with an emphasis on high-resolution electron microscopy (cryo-EM). In the past, we used similar methods to study ribosomes and translation. We are also interested in developing new methods to accelerate and improve cryo-EM structure determination. In particular how we can improve the interpretation of cryo-EM density maps with all-atom models and make these models as accurate as possible.
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