The Evans Lab’s research is focused on the development and clinical translation of optical tools to address challenges in biomedical research and clinical medicine. We use optical spectroscopy, imaging, and microscopy methods to detect, measure, and quantify what is otherwise invisible to address unmet needs in the care of patients.

We take an interdisciplinary, team-based approach in our research, combining physics, chemistry, biology, engineering, computer science, and clinical expertise. Our lab is located at the Charlestown Navy Yard Building 149, a campus that encourages and fosters discovery, interaction, and collaboration. 

Our efforts are focused in three highly complementary areas:

Tissue Oxygen Sensing: “Smart” Bandages and Wearable Devices

We are developing new tools and techniques for detecting, quantifying, and monitoring tissue oxygenation properties. The centerpiece of this technology are a set of newly synthesized, brightly emitting porphyrin oxygen sensors whose emission is modulated by the presence of molecular oxygen. When paired with a green-emitting reference dye, the red phosphorescence emission can be used to precisely measure tissue oxygenation. We have expanded the scope of our tools to include not only tissue oxygen concentration, known as oxygen tension or pO₂, but also metrics such as oxygen consumption rate, pulse rate, and blood oxygen saturation in a single measurement. We are developing molecular and electronic methods to be integrated into sensing films, bandages with drug-release capabilities, wearable sensors for patient monitoring, and sports monitors for recovery and performance training. This technology is currently in three clinical trials in the Boston area, and will soon expand to include new studies as we grow this exciting research direction.

Advanced Microscopy: Coherent Raman Imaging, Nonlinear Absorption, and Lifetime Microscopies
Our laboratory is deeply interested in the development of advanced microscopy tools to address biomedical and clinical problems, with a focus on applications in dermatology.  While coherent Raman imaging is a team favorite, we are “technique agnostic” and both use and develop new imaging methods depending on the research or clinical need. For problems in the area of melanoma research, we routinely use a combination of coherent Raman and nonlinear absorption techniques, such as sum-frequency absorption and pump-probe microscopies. A priority in our advanced microscopy research is the in the growing field of Pharmacokinetic and Pharmacodynamic Tomography, where drugs and their downstream effects are measured and quantified using imaging tools. We use mainly coherent Raman and fluorescence lifetime approaches paired with machine learning to map drug uptake and follow treatment response.

Collaborators within Wellman:   Rox Anderson;  Hensin Tsao;  Mei Wu

External Collaborators:   David Fisher (MGH);  David Langenau (MGH);  Peter So (MIT);  PnP Research Corp;  Manish Patankar (U Wisc);  Daniel Cramer (BWH);  Irene Georgakoudi (Tufts);  Tarl Prow (U Queensland);  Dieter Manstein (CBRC);  Sam Lin (BIDMC);  Rodney Chan (USAISR);  Xiomara Calderon-Colon (Johns Hopkins);  Lloyd Miller (Johns Hopkins);  Christine Huang (MGH)

Selected Publications:

Feizpour, A, Marstrand, T, Bastholm, L, Eirefelt, S, Evans, CL. Label-free Quantification of Pharmacokinetics in Skin with Stimulated Raman Scattering Microscopy and Deep Learning. JID. 2021. 141(2). 3950493. PMID: 32710899.

Pence, IJ, Kuzma, BA, Brinkman, M, Hellwig, T, Evans, CL. Multi-window sparse spectral sampling stimulated Raman scattering microscopy. Biomed Opt. Exp. 12(10). 6095-6114. PMCID: PMC8547998.

Cascales, JP, Greenfield, DA, Roussakis, E, Witthauer, L, Li, X, Evans, CL. Wireless wearable sensor paired with machine learning for the quantification of tissue oxygenation. IEEE IoT. 2021 vol. 8, no. 24, pp. 17557-17567.

Marks, HL, Cook, K, Roussakis, E, Cascales, JP, Grinstaff, M, Evans, CL. Quantitative luminescence photography of a swellable hydrogel dressing with a traffic-light response to oxygen. Adv. Func. Mat. 2022.